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M94A0725.TXT
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1994-10-21
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Document 0725
DOCN M94A0725
TI Mechanisms of AZT resistance in HIV isolates.
DT 9412
AU Buchanan L; Tachedjian G; Birch C; NCHVR, Fairfield Hospital, Victoria.
SO Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:110 (poster no. 70).
Unique Identifier : AIDSLINE ASHM5/94348930
AB To investigate the susceptibility of the reverse transcriptase (RT) and
ribonuclease H (RNase H) from AZT-sensitive (AZT-S) and AZT-resistant
(AZT-R) HIV isolates to AZT-triphosphate (AZTTP) and AZT-monophosphate
(AZTMP). METHODS--Isolates obtained from a patient before and after
long-term AZT therapy were examined for susceptibility to AZT. RT and
RNase H from these isolates were analysed in in vitro assays to
determine their susceptibilities to AZITP and AZTMP respectively.
RESULTS AND CONCLUSIONS--Amino acid changes occurring in the HIV RT
after long-term AZT therapy result in phenotypic AZT resistance.
However, altered susceptibility cannot be definitely associated with the
effects of AZTTP on RT or AZTMP on RNase H using enzyme assays,
suggesting that the assay procedures used are not optimal or that
alternative mechanisms of action are operating for AZT.
DE Antiviral Agents/PHARMACOLOGY Drug Resistance, Microbial/GENETICS
Human HIV/*DRUG EFFECTS HIV Infections/*DRUG THERAPY Long-Term Care
Reverse Transcriptase/ANTAGONISTS & INHIB Thymine
Nucleotides/PHARMACOLOGY Virus Cultivation Zidovudine/ANALOGS &
DERIVATIVES/PHARMACOLOGY/*THERAPEUTIC USE MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).